![]() ![]() The use of flow cytometry enables accurate simultaneous evaluation of multiple analytes, in a statistically relevant sample size in a short time. This application note discusses the use of the Invitrogen Attune NxT Flow Cytometer with Autosampler to evaluate the effectiveness of several cancer drugs in inducing apoptosis in cancer cells, with dose response curves. Jurkat cells, comprising human T lymphocyte cells used to study acute T cell leukemia, were grown and treated in 96-well plates with various concentrations of different cancer drugs as described below. These cells were evaluated for toxicity using an apoptosis reporter assay and analyzed on the Attune NxT Flow Cytometer. Cell toxicity was measured using the fluorogenic substrate Invitrogen CellEvent Caspase-3/7 Green Detection Reagent, which reacts with the enzymes caspase-3 and caspase-7, which are activated during apoptosis. The new color scheme will default to the colors of the current graph, so this makes it easy to transfer a color scheme from one computer to another: Apply the color scheme to a graph on one computer, open the file on another, and create a new scheme based on that graph.Īfter you click the "Define Color Scheme" button shown in the screenshot above, you can also delete any user-defined color scheme you no longer use.The ability to add the CellEvent Caspase-3/7 Green Detection Reagent directly to the live cells in the medium without the need to wash, fix, or permeabilize the cells is ideal for this endpoint assay experiment. Click the Color Scheme toolbar button and choose Define Color Scheme. To create a new color scheme, start from a graph with the colors you like. ![]() You are not limited to the color schemes we provide. In the color schemes dialog, you can choose a color scheme by name using the drop down menu, or use the arrow buttons to browse the schemes. You can choose standard schemes right from the menu, or click More Color Schemes to have more choices. ![]() Then click on the Color Scheme button in the Change section of the Prism toolbar. Go to the graph you want to change, or select several graphs in the gallery. ![]() Prism 8.1 added new color schemes, including a "Colorblind Safe" color scheme, with colors designed to be distinguishable even by individuals with a variety of different color vision deficiencies. Color schemes change all of a graph's colors at once. ![]()
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